2017-8-11 16:0:32025-05-28 22:40:44
locus
BSU02130
BSU_02130
geneLength
879
882
outlinks
bsu
BSU02130
BSU_02130
The protein
Catalyzed reaction/ biological activity
exohydrolytic activity on wall teichoic acid, acts in concert with [[protein|PhoD]] [Pubmed|27780866]
A glycerophosphodiester + H2O = an alcohol + sn-glycerol 3-phosphate (according to Swiss-Prot)
exohydrolytic activity on wall teichoic acid, acts in concert with [[protein|PhoD]] [Pubmed|27780866]
removes glycerolphosphates from the free end of the unsubstituted wall teichoic acid up to the peptidoglycan linker [pubmed|32047114]
sn-glycero-3-phosphoester + H2O --> alcohol + H+ + sn-glycerol 3-phosphate (according to UniProt)
The protein
Protein family
glycerophosphoryl diester phosphodiesterase family (according to Swiss-Prot)
glycerophosphoryl diester phosphodiesterase family (with [[protein|YhdW]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|YhdW]], [[protein|YqiK]]
[[this]]
The protein
Structure
[PDB|4R7O] (the enzyme from ''Bacillus anthracis'')
[PDB|5T9B] [Pubmed|27780866]
[PDB|5T9B] [Pubmed|27780866]
References
Original publications
The protein
[SW|Domains]
GP-PDE domain (aa 38-290) (according to UniProt)
Biological materials
Mutant
BKE02130 (Δ[[gene|glpQ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCTCCTCCTTATA, downstream forward: _UP4_TAAAAGCAAAAAACCTTGCG
BKK02130 (Δ[[gene|glpQ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCTCCTCCTTATA, downstream forward: _UP4_TAAAAGCAAAAAACCTTGCG